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KMID : 0923620120120030118
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Immune Network
2012 Volume.12 No. 3 p.118 ~ p.125
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Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60
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Ryu Su-Jeong
Kang Bo-Ra
Kim Seok-Ho
Kim Tae-Woo
Chang Jun
Choi Eun-Young
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Abstract
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CD40-CD40L-mediated help from CD4 T cells is essential to induce primary CD8 T cell responses specific to the non-inflammatory cell-based antigen H60. In this study, using H60 as a model antigen, we generated recombinant vaccinia viruses (rVVs) expressing the H60 CD8 epitope and investigated whether CD4 help was required to activate the CD8 T cell response specific to the virally expressed H60. The immune response after infection with rVVs expressing H60 was similar to that after immunization with H60 congenic splenocytes, with a peak frequency of H60-specific CD8 T cells detected in the blood on day 10 post-infection. A CD8 T cell response specific for virally derived H60 was not induced in CD4-depleted mice, but was in CD40-deficient mice. These results provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.
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KEYWORD
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Recombinant vaccinia virus, Viral epitope, CD4 help, CD8 T cell response
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